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pan pdk1 site 190 pkcγ thr514 p antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pan pdk1 site 190 pkcγ thr514 p antibody
    Pan Pdk1 Site 190 Pkcγ Thr514 P Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pan+pdk1+site+antibody/Phospho-PKC+(pan)+(gamma+Thr514)+Antibody/10__1128_slash_mcb__00230___16-72-18-52
    Average 93 stars, based on 128 article reviews
    pan pdk1 site 190 pkcγ thr514 p antibody - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Western Blot:

    Article Title: Akt is efficiently activated by PIF-pocket- and PtdIns(3,4,5)P3-dependent mechanisms leading to resistance to PDK1 inhibitors.
    Article Snippet: Mutations leading to inappropriate activation of Akt isoforms contribute to proliferation and survival of a significant proportion of human cancers.. Akt is activated by phosphorylation of its T-loop residue (Thr) by PDK1 (3-phosphoinositide-dependent kinase-1) and its C-terminal hydrophobic motif (Ser) by mTORC2 [mTOR (mammalian target of rapamycin) complex 2].. Potent PDK1 inhibitors such as GSK2334470 have recently been elaborated as potential anti-cancer agents.

    Article Title: Elevated SGK1 predicts resistance of breast cancer cells to Akt inhibitors
    Article Snippet: Anti-(phospho-Akt Ser 473 ) (#9271), anti-(phospho-Akt Thr 308 ) (#4056), anti-(phospho-S6 ribosomal protein Ser 235 /Ser 236 ) (#4856), anti-(total S6 ribosomal protein) (#2217), anti-PTEN (#9559) and anti-phospho-NDRG1 Thr 346 (#5482) antibodies were purchased from Cell Signaling Technology. .. For immunoblotting of the phosphorylated T-loop of SGK1, we employed the pan-PDK1 site antibody from Cell Signaling Technology (#9379) as previously described [ ]. ..

    Article Title: Elevated SGK1 predicts resistance of breast cancer cells to Akt inhibitors
    Article Snippet: Anti-(phospho-Akt Ser473) (#9271), anti-(phospho-Akt Thr308) (#4056), anti-(phospho-S6 ribosomal protein Ser235/ Ser236) (#4856), anti-(total S6 ribosomal protein) (#2217), anti-PTEN (#9559) and anti-phospho-NDRG1 Thr346 (#5482) antibodies were purchased from Cell Signaling Technology. .. For immunoblotting of the phosphorylated T-loop of SGK1, we employed the pan-PDK1 site antibody from Cell Signaling Technology (#9379) as previously described [21a]. ..

    Phospho-proteomics:

    Article Title: Elevated SGK1 predicts resistance of breast cancer cells to Akt inhibitors
    Article Snippet: *MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, U.K., and †Oncology iMED, AstraZeneca, Alderley Park, Cheshire SK10 4TG, U.K. .. Figure S1 Characterization of pan-PDK1-site antibody (CST #9379) for monitoring SGK1 Thr256 phosphorylation HEK-293 cells were transiently transfected with either wild-type, T256A or S422A GST (glutathione transferase)– N-SGK1. ..

    Transfection:

    Article Title: Elevated SGK1 predicts resistance of breast cancer cells to Akt inhibitors
    Article Snippet: *MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, U.K., and †Oncology iMED, AstraZeneca, Alderley Park, Cheshire SK10 4TG, U.K. .. Figure S1 Characterization of pan-PDK1-site antibody (CST #9379) for monitoring SGK1 Thr256 phosphorylation HEK-293 cells were transiently transfected with either wild-type, T256A or S422A GST (glutathione transferase)– N-SGK1. ..



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